DNA Extraction and Purification
New England Biolabs, Inc. NEBNext dsDNA Fragmentase – 250 reactions
NEBNext dsDNA Fragmentase is an enzyme-based reagent that shears DNA to produce fragments of the desired sizes in a time-dependent manner, for next generation sequencing library preparation protocols Note: NEBNext dsDNA Fragmentase is not part of the NEBNext Ultra II FS (Fragmentation System) reagents.
Edge Biosystems Inc PERF. DTR ULTRA 96-WELL PLT KT
fully hydrated gel filtration matrix that provides for a convenient and simple method of dye terminator removal for sequencing cleanup.
Zymo Research Corporation Quick-DNA™ 96 Plus Kit (2 x 96 Preps)
For high yield total DNA extraction (e.g., genomic, mitochondrial, viral) from cell culture, solid tissue, saliva, and any biological fluid sample. Innovative reagents and Zymo-Spin Technology allow for ultra-pure and concentrated genomic DNA (> 50 kb) to be eluted in as little as 10 l. Zymo-Spin Columns ensure no buffer retention. Purified DNA is RNA-free bypassing the need for RNase A treatment and ensuring accurate quantification for applications like library preparations. Isolated DNA is suitable for immediate use in sensitive downstream applications including qPCR, DNA-seq, arrays, and methylation analysis.
CUBE BIOTECH HighSpec Rho1D4 MagBeads Starter Set; 1 set
1ml Highspec Rho1D4 MagBeads + 5mg Highspec Rho1d4 peptide for easy introduction into the work with the Rho1D4-tag
Zymo Research Corporation ZymoBIOMICS™ Lysis Solution, 150 ml
Used in combination with the ZR BashingBead Lysis Tubes, it assists with microbial lysis. It is certified to be low-bioburden to ensure minimal background contaminants for low abundance microbiome samples.
Norgen Biotek Corp Plasma/Serum Cell-Free Circulating DNA Purification Midi Kit
This kit provides a fast, reliable and convenient method to purify and concentrate using a two column method for the isolation of high quality, high purity and inhibitor-free cell-free circulating DNA (cfc-DNA) from fresh or frozen plasma/serum sample volumes ranging from 1 mL - 4 mL.The first column will handle the large volume input of bodily fluids that is followed by a concentration on a mini column for a final elution of 25 µL to 50 µL. All components for the purification & concentration are provided in one convenient & fast kit for the easy processing of large input volumes of bodily fluids.The purified plasma/serum cfc-DNA is fully compatible with all downstream applications including PCR, qPCR, methylation-sensitive PCR and Southern Blot analysis, microarrays and NGS, for various analyses: Fetal DNA screening, genotyping, and other oncology and autoimmune diseases research.
Zymo Research Corporation Quick-DNA™ Viral Kit (50 Preps) w/ Zymo-Spin™ IC Columns (Capped)
Quick recovery of viral DNA from a wide range of sources using Zymo-Spin column technology. Column design allows DNA to be eluted at high concentrations into minimal volumes. Eluted DNA is suitable for PCR, Southern blotting, and restriction endonuclease digestion.
Zymo Research Corporation ssDNA/RNA Clean & Concentrator™ (50 Preps)
This simple procedure is based on the use of a unique single-buffer system and Zymo-Spin™ Column technology. Single stranded DNA or RNA (17 to 200 nucleotides; e.g., short transcripts, probes, primers) can be safely treated and recovered using this kit. The result is highlyconcentrated ( 6 l), purified DNA/RNA that is suitable for subsequent molecular methods including PCR, RT/PCR, hybridization, etc.
New England Biolabs, Inc. NEBNext dA-Tailing Module – 20 reactions
This module is part of the original standard library prep workflow, which is longer than the NEBNext Ultra II DNA Library Prep Kit workflow, has more components, and requires more clean-up steps. It also requires higher input amounts: 1 - 5 µg of input DNA vs. 500 pg - 1 µg required for the Ultra II DNA workflow.
Zymo Research Corporation ZR BashingBead™ Lysis Tubes (2 mm) (50 tubes)
Each impact resistant 2 ml tube contains 0.7 ml dry volume 2.0 mm ZR BashingBead™ lysis matrix. The state of the art, ultra-high density beads are fracture resistant, chemically inert, and ideal for disrupting tough-to-lyse biological samples. These beads are ideal for tissues, insects, plant material, etc.